Cellular physiology platform – CRCHUM (animal/human)
Name of entity
Cellular physiology platform - CRCHUM (animal/human)
Entity type
Platform: Cytometry/imaging/microscopy
Keywords
Insulin, c-peptide, glucagon, cell mass quantification, morphometry, ELISA, AlphaLISA, beta cell mass, immunohistochemistry, cellular imager, SeaHorse, Operetta, proliferation, apoptosis, oxygen consumption.
Area of expertise
Our mission is to support research teams (universities and industry) in the fields of cell physiology and biochemistry by providing state-of-the-art quantification services using advanced instrumentation with very competitive prices. More specifically, the core facility is specialized in the quantification of analytes, hormones cytokines, etc, found in plasma/serum samples by ELISA and AlphaLISA, as well as in tissues by immunohistochemistry. The core facility has also a Seahorse instrument for measuring glycolysis flux and mitochondrial respiration as well as a high content cell imager (Operetta from PerkinElmer) to monitor cellular phenotypic changes using fluorescent dyes (proliferation, apoptosis, migration, translocation etc).
Services offered
Metabolism-related determinations
Immunoassay & biochemical determinations
Quantification of hormones, cytokines and peptides (insulin, c-peptide, glucagon, GLP-1, TNFa, leptin, IL-6, etc) in plasma/serum and incubation media via ELISA and AlphaLISA.
Measurement of beta & alpha cell masses by immunohistochemistry
Quantification of beta and alpha cell masses, b cell / a cell ratio, cell proliferation (Ki-67) and apoptosis (TUNEL) by immunohistochemistry as well as morphometry measurements using an Aperio slide scanner.
Seahorse XF24-3 flux analyzer.
The Seahorse XF24-3 instrument analyzes mitochondrial respiration and glycolysis in live cells. More specifically it measures oxygen consumption, CO2 production and extracellular acidification using special detectors (label-free assays).
Operetta high content imaging system
Measurement of various cellular activities by high content fluorescence imaging using the Operetta imager. The Operetta allows the quantification of cell activities such as apoptosis, proliferation, migration, intracellular protein translocation, mitochondrial mass & activities in 96/384 well plate format, in confocal or non-confocal mode.
Responsable
Erik Joly
erik.joly@crchum.qc.ca
514-890-8000 poste 23608
http://crchum.chumontreal.qc.ca/en/core-facilities-and-services/metabolomics-and-cellular-physiology
Address
Erik Joly, PhD
Associate Director -Cores & Development
Montreal Diabetes Research Center
CHUM research center –Tour Viger
900, rue St-Denis- Bureau R12-464
Montreal, Québec
H2X 0A9