Metabolomics platform – MHI (animal/human)

Name of entity

Metabolomics Platform - Montreal Heart Institute (MHI) (fundamental/clinical)

Entity type

Platform of collaboration and service: Mass Spectrometry / Metabolomics

Keywords

Metabolism, metabolomics, targeted and untargeted lipidomics, metabolic flux, stable isotopes, mass spectrometry, GC-MS, LC-MS, LC-QQQ, LC-QTOF.

Area of expertise

Our primary research focus is on cardiovascular, cardiometabolic, mitochondrial and inflammatory diseases. We can do absolute and/or relative quantitative, targeted or untargeted, analyses of metabolites, including their labeling with carbon 13 or deuterium) both in fundamental and clinical projects. We also analyze various classes of metabolites in different kind of biological matrices such as plasma, serum, red blood cells, cells, mitochondria, or various animal tissues (heart, liver, muscle, brain and more) and other species (ex: C. elegans) depending on your needs. We can process all analyses methods on a new matrix after achieving feasibility tests.

Services offered

1) Available analysis using validated methods.

Untargeted analyses (relative quantification):

Untargeted lipidomic analysis ( >1500 reproducible entities; >500 unique lipids) by LC-QTOF.
Data analysis on chosen class.

Targeted analyses:

Quantitative analysis of amino and organic acids by GC-MS (33 metabolites).
Quantitative analysis of fatty acids including saturated, monounsaturated, polyunsaturated (omega-3 and omega-6) and very long chain fatty acids by GC-MS, in specific fractions (phospholipids and triglycerides) on demand (29 metabolites).
Semi-quantitative analysis of acylcarnitines by LC-QQQ (C2 à C26; >50 isoforms).
Quantitative analysis of metabolites stemming from glucose metabolism such as phosphate sugars and UDP-N-acetyl-glucosamine by LC-QTOF.
Metabolic flux analysis related to energy metabolism (glycolysis; substrate oxidation).
Trimetylamine oxyde (TMAO) and associated metabolites.

2) Method development on demand. 3) Scientific support (study design and data interpretation).

Directeur

Director : Christine Des Rosiers, Ph.D. and co-director : Matthieu Ruiz, Ph.D.

Responsable

Isabelle Robillard Frayne, M.Sc. and Caroline Daneault, M.Sc.
metabolomique.icm@mhi-rc.org
514 376-3330 poste 2133
https://www.icm-mhi.org/en/research/labs/fundamental-laboratories/metabolomics

Address

Metabolomics Platform – Montreal Heart Institute (MHI) Montreal Heart Institute Research Center, S-3730 5000, Bélanger street Montreal, Québec H1T 1C8